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With the intention to estimate the potential metabolism of L-hydroxyproline, P. aeruginosa PAO1 was cultivated in minimal medium supplemented with L-proline, trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, or cis-3-hydroxy-L-proline as the sole carbon supply. L-Hydroxyproline metabolism in P. aeruginosa PAO1. Concentrations of L-hydroxyproline in medium were measured using an amino acid analyzer. We herein demonstrated that PaLhpL gene (PA1255; […]
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We observed 4 prominent bands in these four protein extracts and their band assignments are as follows: band near 1678 cm?1 assigned to irregular construction (random coils)40, band near 1660 cm?1 assigned to alpha helices and irregular structure10, band near 1642 cm?1 assigned to irregular structures and beta sheets38, band close to 1628 cm?1 assigned […]
Well, as it seems, this side chain has a pKa of around 6.5. And this seems to be really close to physiological pH, which is correct around 7.4. So what does this really imply– to have a pKa that’s close to physiological pH? Atomic labels of 1 asymmetric unit have been proven for non-H atoms. […]
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Preparation, stability, and photoreactivity of thiolato ruthenium polypyridyl complexes: Can cysteine derivatives protect ruthenium-based anticancer complexes? The behaviour of the complexes in water and cell culture medium was investigated by multinuclear NMR and UV-Vis spectroscopy, revealing a considerable influence of the monodentate ligand on the aqueous chemistry. Khalid, K. Ali. 2006. Influence of Water Stress […]
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